Latino Studies at New York University

David Gresham

NYU Center for Genomics and Systems Biology

October 18, 2011

Global Analysis of Cell Growth Programs in Saccharomyces cerevisiae

Cells regulate their rate of growth in response to extracellular conditions.  We have studied control of cell growth by yeast cells (Saccharomyces cerevisiae) in nitrogen-limited conditions using chemostat (i.e. continuous) cultures.  Steady-state analyses enabled quantification of growth-dependent and -independent gene expression differences.  By perturbing steady-state cultures through pulsed addition of nitrogen we studied the kinetics of global gene expression remodeling in cells transiently relived of nutrient limitation.  We find evidence that accelerated mRNA degradation plays an important role in the transition between gene expression states.  Our studies provide new insights into how the cell coordinates molecular functions with regulated cell growth and quiescence.